RE: SPAM-LOW: [Histonet] MiTF on a Ventana Immunostainer

From:"Douglas D Deltour"

We just started using the MITF-1 antibody on the XT. I got it validated and
we were running it for about 2 months and then all of a sudden it stopped
working, but that is another story. Which company did your colleague get the
antibody from? I used the same protocol that I used for the MART-1 for
validation. This was working great for the MITF until.....

Douglas D. Deltour HT(ASCP)
Histology Manager
Professional Pathology Services, PC
One Science Court
Suite 200
Columbia, SC 29203
Office (803)252-1913
Fax (803)254-3262 
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-----Original Message-----
[] On Behalf Of Sate Hamza
Sent: Sunday, November 18, 2007 10:01 AM
Subject: SPAM-LOW: [Histonet] MiTF on a Ventana Immunostainer


One of my colleagues posted the following inquiry on another forum.

"Does anyone have a protocol for using MITF-1 on a Ventana immunostainer?
Ventana doesn't have the antibody so they are not much help in either
acquiring the antibody or helping us get started using it."

I was wondering if anyone is using MITF on a Ventana Immunostainer and would
be able to help.

Many thanks in advance.


Sate Hamza, MD, FRCPC
Winnipeg, Canada
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