RE: [Histonet] Uranyl nitrate disposal

From:"Bartlett, Jeanine (CDC/CCID/NCZVED)"

We are not allowed to dispose of our uranyl nitrate in the normal
hazardous waste.  Our radiation group handles it. 

Jeanine Bartlett, BS, HT(ASCP)QIHC
Centers for Disease Control and Prevention
Infectious Diseases Pathology Branch
1600 Clifton Road, MS/G-32
Atlanta, GA  30333
(404) 639-3590

-----Original Message-----
[] On Behalf Of Philip
Sent: Thursday, November 29, 2007 1:34 PM
Subject: [Histonet] Uranyl nitrate disposal

Uranyl nitrate and Uranyl acetate are routinely used as stains for
transmission electron microscopy (really contrasting agents, not stains
in the light microscopy sense). Both for sections and negative stains of
bacteria, viruses, and macromolecules.
Waste disposal is just as a toxic compound (uranium is toxic) in water.
The radioactivity is too low to bother about, even for the safety people
-- although there are probably some safety offices that regulate UNO3
and UAc they same way they do carbon-14 or phosphorus-32, which are


>The preferred name for this venerable silver technique is "reticulum 
>stain". Whatever reticulin is (or was), it isn't all that the technique

>demonstrates. I never heard anybody say "reticular stain".
>How are people handling the hazmats problems with reticwhatever stains?

>Is anybody still using uranyl nitrate?
>Bob Richmond
>Samurai Pathologist
>Knoxville TN
>Histonet mailing list

Philip Oshel
Microscopy Facility Supervisor
Biology Department
024C Brooks Hall
Central Michigan University
Mt. Pleasant, MI 48859
(989) 774-3576

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