[Histonet] re: macrophages/microglia in FFPE tissue
Hi Zach.
Perhaps you need to try heat-induced epitope retrieval/unmasking on you pwax
sections?
I have tried an OX-42 Ab reagent on FFPW sections after the above with
negative results. ( and tried various proteolytic enzmes). The only
excellent result , for me, was after HIER on pwax rat brain, using an
anti-phosphotyrosine Ab reagent. See here for your evaluation;
http://www.immunoportal.com/index.php Go to Image gallery and use search box
"phosphotyrosine". No guarantees for your species, of course.
Good luck.
carl
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