RE: [Histonet] automated immuno stainer

From:"Patsy Ruegg"

I agree with Patti.  
I have used Ventana, Biogenex and Dako IHC stainers in research and clinical
settings.  For cost effectiveness, consistent, reliable results I choose the
Dako.  I have had the same Dako rep for over 10 years and she is great
(LuAnne you know who I am talking about).  I did not have to pay for the
shipping of my instrument and use the instrument based on reagent
requisition.  We worked together for weeks validating the antibodies I use
and they even provided the reagents in some cases.  I know Dako has had some
troubles of late, but for my money and satisfaction they have and continue
to serve me well.
Patsy Ruegg 

-----Original Message-----
[] On Behalf Of
Sent: Monday, November 27, 2006 8:49 PM
To: histonet; Patti Loykasek; Chistine Tambasco
Subject: Re: [Histonet] automated immuno stainer

I've used the Ventana, Biogenex and Vision Biosytem Bond IHC stainers. The
Vision and Ventana are more user friendly for online dewaxing and antigen
retrieval but I really did like the RTU antibodies from Biogenex.They were
very consistent and clean staining.  Vision Biosytem (Steve Westra in
Florida) did have the best technical support though.
At one place I worked, the Dako rep wanted to charge us $1,700.00 to ship a
demo IHC stainer for us to try. My MD didn't even consider Dako after that.
There is no perfect system. You just need to find the one that will fit your
needs and budget. I would suggest getting a solid price quote on the
equipment, antibodies and other disposable products prior to purchasing.

Ron Martin, BS, HT (ASCP) HTL, QIHC 
---- Patti Loykasek  wrote: 
> Hi Christine. I believe that a good, well trained tech can achieve good
> immunos on any type of platform. I think the main issues are your
> preferences: do you want an open or closed system - any detection system
> only the instrument company, concentrated or prediluted antibodies,etc?
> Antigen retrieval on instrument or ok off instrument? Cost per slide? Ease
> of waste disposal? Can you use the clones of antibodies you have currently
> validated on the instrument? User friendly? Those are the main issues I
> think of at the moment - my brain is still tired from all the turkey
> tryptophan!  
> Most companies are more than happy to provide you with a demo & they may
> even help work up some things for you.
> As for my preference - it is Dako. We have several of the 48 slide
> configured Autostainers. I'd be happy to respond to any questions you
> have about the Dako.
> Good luck in your stainer hunting.
> Patti Loykasek BS, HTL, QIHC
> PhenoPath Laboratories
> Seattle, WA
> > Hello all! I hope everyone enjoyed their breif repreive from stress. I
> > wondering if anyone could give me some input on automated stainers for
> > immunohistochemistry. Currently we hand stain and our volumes are
> > increasing. We are looking at automation as we probably do up to 12-15
> > per day and plan on starting ER and PR. I heard Vantana is very good.
> > about Leica?  (Theirs does both immunos and ordianry special stains.)
> > have any experience they would like to share? We are an Ascension Health
> > affiliated hospital as that may matter in the negotiations. Thanks for
any and
> > all opinions.
> > Respectfullly,
> > Christine Tambasco, HT (ASCP)
> > St. Mary's Hospital
> > Amsterdam, New York 12010
> > 518-841-7287
> > _______________________________________________
> > Histonet mailing list
> >
> >
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