RE: [Histonet] Re: nuclear bubble artifact

From:"Marshall Terry Dr, Consultant Histopathologist"

Firstly, they are not nuclear, but often more conspicuous in nuclei because of contrast.

You do what I have spent countless hours doing - changing times reagents and anything else that you can think of.
Heat is invariably mentioned as a cause, and it certainly may be a causative factor, but although when Kemlo and I originally tackled this years ago, we eliminated it by cooling everything down, this did not work when I was in Oman, and spent countless hours fiddling.
The crux of the matter is to find out what the bubbles are due to. For sure, it's something that is or has been beneath the section. For sure it's not a stray Calorie or two.
I say section, but I have seen them in cytology preparations as well as frozen sections, trashing the sometimes suggested cause of residual wax.

I have a theory on which I may work, but I'm keeping it close to my chest.
How much is a Nobel prize worth?

Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path
 Consultant Pathologist
 Rotherham General Hospital
 South Yorkshire

-----Original Message-----
From: []
Sent: 08 November 2006 18:14
Subject: [Histonet] Re: nuclear bubble artifact

I know this subject has come up before, and I may have missed it on Histonet, 

How do you trouble shoot nuclear bubble artifact? 

Nuclear bubble artifact really degrades pathologic diagnosis. I've never been 
clear how to advise a histotechnologist on how to look for the cause and fix 
it. Can some of the senior histotechnologists on HIstonet address this issue 
for all of us?

Bob Richmond
Samurai Pathologist
Knoxville TN
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