My name is Stephen Clark and i work in the neuro lab at Eastern Illinois University. We use fluorescent immunohistochemistry to stain the Olfactory Epithelium of mice and have recently had a problem with autofluorescence of our tissues. They are fixed in 4% paraformaldehyde in PBS and i have already tried sodium borohydrate with little success. I have read about photobleaching using UV, neon, and lights of specific wavelengths (488 and 633nm). I am just wondering if anyone utilizes photobleaching via a light source and where it would be possible to purchase them. I am also wondering if the 18um sections might be a little too thick and whether that would have an increased affect on autofluorescence. Any tips would be appreciated.
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