Re: [Histonet] H&E Staining of Frozen Sections

From:Rene J Buesa

We used to follow the following protocol:
  1- fix the frozen section  in acetone X 10 secs.
  2- distilled water x 10 secs
  3- hematoxylin x 30 secs
  4- tap water
  5- quick dip in acid alcohol
  6- eosin 5-10 secs.
  7- 95% ethanol > 100% ethanol x2 > xylene X2 > coverslip.
  The pathologists had the slide ready in about 2-3 minutes.
  Rene J.

Rebecca Jo  wrote:
  Hi Histoneters-

I was wondering if someone could give me a protocol for H&E staining on frozen
sections. I assume I can just skip over the deparaffinizing steps and go
straight to the staining, but I wanted to know how long I should keep the
sections in hematoxylin. Just enough to stain the sections? Any help would
be greatly appreciated.


Rebecca E. Jo
UNC-School of Medicine
Department of Cell and Developmental Biology
CB# 7090

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