[Histonet] H&E Staining of Frozen Sections

From:Rebecca Jo

Hi Histoneters-

I was wondering if someone could give me a protocol for H&E staining on frozen
sections.  I assume I can just skip over the deparaffinizing steps and go
straight to the staining, but I wanted to know how long I should keep the
sections in hematoxylin.  Just enough to stain the sections?  Any help would
be greatly appreciated.


Rebecca E. Jo
UNC-School of Medicine
Department of Cell and Developmental Biology
CB# 7090

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