RE: [Histonet] cloth yellow stain

From:"Rittman, Barry R"

Is it possible that this is a Procion dye (chloro- -s triazine)?
these have ben used extensively to dye fabrics and still are used for this purpose.


From: on behalf of Paul Bradbury
Sent: Tue 11/15/2005 7:20 PM
To: McCollough, Carol; HistoNet Server
Subject: Re: [Histonet] cloth yellow stain

For the last couple of hours I have been racking my brain to try to
recall where I have come across this dye before.
But, the required neurons are not responding ... or only weakly.

John Kiernan is right on target ( as usual) in suggesting that this dye
is almost certainly a large molecule azo dye (actually a very long,
linear molecule) anionic dye probably used in the textile industry for
coloring cotton fibres. If I remember correctly, such dyes are often
referred to as "milling dyes". Similar azo dyes are commonly used for
dying natural fibres in industry. In histology, dyes of this type are
used to show amyloid (Congo red being the classical example).

Yellow dyes provide poor visibility to tissue elements, so this dyes is
almost certainly a counterstain used to highlight general background
structures and to contrast with a red or blue primary stain. My
suggestion would be to make up a 0.5% solution of the dye in 1% acetic
acid, and try it out on a few sections of tissue.

Let us know what the results are ... I am sure we would all love to know
for sure..

Paul Bradbury
Kamloops, BC

McCollough, Carol wrote:

>Greetings Histonetters:
>In an exhaustive inventory of laboratory chemicals we have discovered an old bottle of 'Cloth Yellow' stain powder, manufactured by Chroma.  Efforts to get an MSDS from Chroma have failed.  Nobody here knows what this was even used for.
>Would anyone care to offer any histological uses for this stain?
>Regards -
>Carol B. McCollough
>Aquatic Animal Research Pathologist
>Oyster Disease Research Project
>Fisheries Service
>Maryland Department of Natural Resources
>Cooperative Oxford Laboratory
>904 S. Morris Street
>Oxford, Maryland 21654
>410-226-5193 x124
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