You may be over heating your samples for cd31, I had to go to a waterbath at
80dc for 3 hours using the low ph hier buffer.
Patsy 


Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 216
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
wk email pruegg@ihctech.net
web site www.ihctech.net
 

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-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Histology
SLU
Sent: Thursday, November 17, 2005 7:10 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] CD31 staining histiocytes???

Hello all:
   
  Is anyone out there using Dako CD31 and getting histiocyte staining in
human skin???  Our dermpath is seeing this and is questioning it.  We are
using it at 1:100 with the biocare pressure cooker with Biocare Diva
decloaker solution.  Our detection system is Envision +.  Any thoughts???
   
  Susan
  Histopathology Supervisor
  St. Louis University Medical School

		
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