RE: [Histonet] Slide Adhesion;toenail for fungus stain

From:"Patsy Ruegg"

It is really tuff to get toe nails and cartilage to stay on the slide,
especially if you use heat when staining.  My very best method includes
pre-coating slides with a 5% elmers glue solution, let the slides airdry
very well after coating, I usually airdry overnight.  Use these slides to
pick up your sample and then airdry them with the section on them again
overnight.  To melt the paraffin I place the airdryed sections on a flat
slide warming plate at 55dc for 10 min., followed again by complete

Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 216
Aurora, CO 80010
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-----Original Message-----
[] On Behalf Of Christine
Sent: Thursday, November 17, 2005 11:15 AM
Subject: RE: [Histonet] Slide Adhesion;toenail for fungus stain

   Dear John,

   I  have  a  lot  of  trouble  getting  toenails  to stay on the slide,
   especially  doing  the GMS stain for fungus. I have used Poly-L-Lysine
   coated  slides,  probe  slides  and  even Elmers glue. I would be very
   interested in a slide that could keep the toenail attached. Thanks for

   Christine Tambasco, HT (ASCP)

   St. Mary's Hospital Laboratory

   Amsterdam, New York 12010


     From:  John Robertson 
     Subject:  [Histonet] Slide Adhesion
     Date:  Wed, 16 Nov 2005 08:42:57 -0500
     >We are interested in seeing if a new slide coating method has
     >advantages  for  the  adhesion  of "difficult" sections. It should
     >well  in  all  applications  and  ultimately  be  less costly than
     >  charged slides.
     >If  you  have  an  especially difficult adhesion problem , I would
     >to  hear  from  you  and  offer  you  a  few prototype samples for
     >John Robertson   P.E. Ph.D.
     >CEO  InfoSight Corporation     "
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