[Histonet] best perfusion solution for spinal cord
I was hoping you guys could help me with something. I have a friend
who is using rat spinal cord injury models. He needs a good method
for fixing the spinal cord to examine both morphology of the spinal
cord tissue and various proteins by IHC. What is the best method of
fixation for this? I assume that what is best for the brain is
usually best for the spinal cord. I recently read that some use a
sucrose solution in their perfusion protocol. I have always just
dropped the brain into 25% sucrose overnight to cryoprotect. I never
considered using it in the perfusion.
Any help would be appreciated. I am particularly interested in which
formulations of fixatives are best and why. For example, could NBF
be used? I mostly see paraformaldehyde in use, usually 2 or 4%.
What are the advantages of this over NBF?
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