[Histonet] how to get this cryostat working?
I have located a cryostat that someone is willing to let me use. It
was inherited from another lab and has been moved to a new building
and has not been powered up for several months. Does anyone have
advice on how I should proceed with getting this cryostat in working
order? It appears to be fairly clean inside and was decontaminated
before it was left to sit. It is a Shandon Cryotome Motorised
Electronic cryostat (no. 77210160 GB).
I will probably use the cryostat initially about once a week. Should
the cryostat be powered on constantly? Or should I turn it on prior
to each use? Should it be kept at a particular temperature between
uses? The cryostat has a waste bottle that collects water from the
cooling coils and also debris from washing the chamber. It is
suggested that this bottle should always contain 10% formalin, I
assume to serve as a decontaminant. Does this always have to be in
use, and should I change the bottle often?
Where can I find the correct type of disposable blades to use with
this cryostat model. I plan to section brain, spinal cord and
liver. Is there a particular brand or type of disposable blade I
should use for this purpose? How often should the blades be changed?
I have used a cryostat before but I haven't been responsible for its
upkeep beyond regular cleaning after use, so if there is anything
that I am missing, or any suggestions, please let me know.
By the way, as far as cryostats go, how does this model stack up?
Any and all suggestions are appreciated.
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