[Histonet] double staining in frozen tissue
Could someone be so kind to share or advise me on a
protocol for double staining immunofluorescence on
frozen human bladder tissue?
1)Would it cause problem if I use FITC donkey
anti-goat IgG and Alexa 647 goat anti-rabbit together
as my secondary antibody?
2)Which fixation is better? I had tried 4%
fornaldehyde (at room temp) for 5 min and icy acetone
(-20) for5 min, and found with acetone-fixation, the
morphology of nucleus is very poor. Any suggestion?
Any help will be highly appreciated,
Department of Epidemiology
School of Public Heath
University of California, Los Angeles
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