Re: [Histonet] F4/80 on formalin fixed frozen spleen
I've had good results with F4/80 staining on formaldehyde fixed
cryostat sections of mouse brain.
However, it did require microwave/citric acid antigen retrieval.
Trypsinisation pre-treatment also works, but I found it to be harsher
on the sections than microwaving.
Division of Neuroscience
University of Edinburgh
1 George Square
Edinburgh EH8 9JZ
T: 0131 6511 875
> Message: 6
> Date: Wed, 24 Nov 2004 13:40:40 -0500
> From: Sharon Cooperman
> Subject: [Histonet] F4/80 on formalin fixed frozen spleen
> To: email@example.com
> Content-Type: text/plain; charset="us-ascii" ; format="flowed"
> Does anyone know if F4/80 can work on formalin fixed frozen mouse
> spleen for IHC without antigen retrieval?
> Also, thanks to everyone for their suggestions re. mouse B-cell
> Sharon Cooperman
> NIH, NICHD, CBMB 301.435-8417
> Building 18T, room 101 301.402-0078 fax
> Bethesda, MD 20892
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