[Histonet] RE: IHC on tissues processed for EM
There are hundreds, if not thousands of papers demonstrating successful localization of intracellular antigens at EM or LM level using tissue samples processed conventionally for EM (i.e. PFA/GA fixation, Os postfixation, epoxy resin embedding).
Look for the now classical papers by Roth and Bendayan or their early reviews. Also the EM textbooks published in the 80's have plenty of information.
And let me also add a personal comment of frustration: Histonet used to be about sharing experience and wisdom, mentoring and teaching those have were (are they still?) eager to learn or perfect their skills. I personally learnt/benefited from many messages posted. Now people are asking advice about coverslip sizes? So thanks to the curse of the Internet, journal articles and text books are used only by a dedicated few, electronic databases (libraries, PubMed, journal archives, reagent search sites, even Google) are not searched, and now even the vendor catalogs are not opened, because on the Histonet somebody will respond? How will users differentiate between solid information (based on scientific knowledge, practical experience) or just plain ignorance? Where is critical thinking? What happened to a once honorable guild of skilled craftsmanship, when members of the community are lacking elementary knowledge (just a few recent hair-raising topics: H&E staining, pH, and the list goes on and on)? And of course we/they are constantly offended/complain about the lack of recognition by our/their peers.
Feel free to get angry with me. I certainly welcome arguments and criticism.. As for me, I go back to find something useful in one of my textbooks.
FROM: László G. Komuves PhD
Senior Principal Scientist,
Manager, Microscopy Core Laboratory
650 Gateway Blvd., South San Francisco, CA 94080
Phone: (650) 246-6905, Fax: (650) 624-7540
Date: Tue, 23 Nov 2004 08:02:24 -0500
From: Michele French
Subject: [Histonet] IHC on Sections Processed for EM?
Content-Type: text/plain; format=flowed; charset=ISO-8859-1
Good Morning Histonet! A colleague of mine did some EM work and found
something interesting. Our pathologist wanted me to try to do some IHC
to further characterize what is present in the section. Our EM person
said it would never work. I did not think it was possible either, but I
thought I would ask anyway. Has anyone tried doing an immunostain on
plastic (Epon) sections from tissue fixed and processed for EM? I am
always up for a challenge, but I am really busy right now and don't want
to waste my time if there is really no hope! Thanks in advance, Michele
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