Does anyone have advise on how to freeze cells that have been spun down 
in a micro centrifuge tube? In the past I've snap frozen
the tube, loosened the cells by flicking the tube and then embedded the 
pellet in OCT. The cells  look OK, but the cells in the middle don't 
look as good as the ones on the perimeter.Any advise would be appreciated.
Thanks,
Sue Wells

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