[Histonet] re: HELP!!! - Autofluorescence in the mouse brain
Sounds like you might be seeing lipofuschin granules, common
autofluorescent intracellular structures in brain. To reduce
Baschong W, Suetterlin R, Laeng RH. Control of autofluorescence of
archival formaldehyde-fixed, paraffin-embedded tissue in confocal laser
scanning microscopy (CLSM). J Histochem Cytochem. 2001 Dec;49(12):1565-72.
They describe studies using ammonia-ethanol, borohydride, and
sudan Black B alone or in combination, with a variety of fixation and
section prep methods compatible with immunolabeling. You can find other
literature regarding the application of these reagents to
autofluorescence problems. Expect some trial-and-error to figure out
what works best for your particular specimens.
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