Re: [Histonet] ISH on cryosections of adult mouse heads

From:John Kiernan

Yes. Disodium EDTA. It's in all the
techniques books more recent than
about 1955.
            John Kiernan
            London, Canada.
-----------------------------------
Tobias Mätzig wrote:
> 
> Hello everyone,
> 
> I'd like to performe ISH an mouse-head cryosections.
> My problem is that I have to decalcify the head before I can cut it.
> Has anyone a good suggestion how to fix and decalcify the adult mouse head without destroying the RNA ?
> 
> Thanks
> 
> -Tobias-
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