I use TritonX to permeablize cells that have been cultured for staining. 
Tween I use as a wetting agent in buffers when washing slides this is what
makes the wash buffer sheet off the slide. I think?!@
c

Cynthia Favara
NIAID/NIH/RML/LPVD
903 South 4th Street
Hamilton, MT 59840
406-363-9317


-----Original Message-----
From: Ryan Kerney [mailto:kerney@fas.harvard.edu]
Sent: Tuesday, November 18, 2003 8:04 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Triton vs. Tween


Can anyone out there tell me when you are supposed to use tritonX instead of

tween 20 and why some protocols use both? Are they both just mild
detergents? 
What is the difference?

thanks, 

Ryan

-- 
Ryan Kerney
Department of Herpetology 
Museum of Comparative Zoology
26 Oxford St. 
Cambridge, MA
02138

Office: 617-496-4065
Lab:    617-496-4632







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