Fwd: [Histonet] Eosinophil stains for mouse tissue
Judi,
Recently
this has been the bane of my life, eosinophils in mouse tissue. Name it
and I've tried it. Carbol chromotrope, lovely on other species. Various
combinations of eosin, strong, weak, long staining, short staining.
Luna's eosinophil granule stain, biebrich scarlet. For me, the best
technique by far for mouse tissue is, Sirius Red (Llewellyn,B.D. 1970. J.
Med. Technol. 27. 308-309). Works a treat every time. All it needs
is a light Haematoxylin and you have lovely stain. I would try immuno but
I'm having a bit of bother finding an antibody, anybody suggest a
source?
Ian.
Hi everyone,
We are looking for a stain which will emphasize the eosinophils in mouse
heart and lung tissue. Any help is greatly appreciated.
Thanks,
Judi Ford
Jackson Laboratory
Bar Harbor, ME
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Dr. Ian Montgomery,
Histotechnology,
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