RE: [Histonet] DBA staining for parietal cells
(Dolichos biflorus) binds to several types of N-acetyl glucosamines. I would go
to two sources.
for a history of lectins "The Lectins. Properties, Functions and Applications in
Biology and Medicine" by Liener, Sharon and Goldstein. Academic Press.
Published in 1986. Although this is an older text it is a excellent resource for
information on all the lectins known up to that time.
Another great sources is Vector Laboratories, I think
still at Burlingame, CA.. They have a booklet that provides a lot of information
re lectins. They do sell lectins and lectin antibodies and all those I have
purchased from them have been outstanding.
are going to use a lectin bound to a peroxidase then these do often have a
limited life span.
DBA and many other lectins there are a number of isolectins with slightly
different binding properties and specificities.
lectins bind to only one carbohydrate moiety and therefore the carbohydrate you
use for negative controls must take this into account.
Negative controls using specific (and highly purified
carbohydrates) are critical in evaluating lectin binding.
MD Anderson Blvd.
Houston, TX. 77030
First of all I want to thank you all for helping
me with my T and B cell staining problem. I got many replies and am working
out some new protocols now. Thanks!
I also have a new question, has someone
already worked with the lectin DBA (horse gram) to color parietal cells?
Could you give me some more information about
that (what are the bindingplaces of this lectin on the parietal cell?) I have
not worked with lectins yet.
Manuelle De Bock, dierenarts (DVM)
of Veterinary Pathology
Department of Pathology, Bacteriology and Avian
Faculty of Veterinary Medicine
B9820 Merelbeke - Belgium
Fax 0032(0)9 264 7789
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