RE: [Histonet] DBA staining for parietal cells

From:"Barry R Rittman"

DBA (Dolichos biflorus) binds to several types of N-acetyl glucosamines. I would go to two sources.
First for a history of lectins "The Lectins. Properties, Functions and Applications in Biology and Medicine" by Liener, Sharon and Goldstein.  Academic Press. Published in 1986. Although this is an older text it is a excellent resource for information on all the lectins known up to that time.
Another great sources is Vector Laboratories, I think still at Burlingame, CA.. They have a booklet that provides a lot of information re lectins. They do sell lectins and lectin antibodies and all those I have purchased from them have been outstanding.
Some cautions
If you are going to use a lectin bound to a peroxidase then these do often have a limited life span.
For DBA and many other lectins there are a number of isolectins with slightly different binding properties and specificities.
Few lectins bind to only one carbohydrate moiety and therefore the carbohydrate you use for negative controls must take this into account.
Negative controls using specific (and highly purified carbohydrates) are critical in evaluating lectin binding.
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-----Original Message-----
From: [] On Behalf Of Manuelle De Bock
Sent: Friday, November 14, 2003 2:15 AM
Subject: [Histonet] DBA staining for parietal cells

Hello everybody,
First of all I want to thank you all for helping me with my T and B cell staining problem. I got many replies and am working out some new protocols now. Thanks!
I also have a new question, has someone already worked with the lectin DBA (horse gram) to color parietal cells?
Could you give me some more information about that (what are the bindingplaces of this lectin on the parietal cell?) I have not worked with lectins yet.
Manuelle De Bock, dierenarts (DVM)
Laboratory of Veterinary Pathology
Department of Pathology, Bacteriology and Avian Diseases
Faculty of Veterinary Medicine
Ghent University
Salisburylaan 133
B9820 Merelbeke - Belgium
Tel 0032(0)9 264 7745
Fax 0032(0)9 264 7789

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