Greetings, Histonetters.

One of my pathologists has expressed concern that antigenicity for certain antibodies, specifically ER and PR, in breast tissue that is processed using alcohol-formalin will be decreased.  Can anyone cite a reference in the literature that confirms or addresses this concern?  Has anyone had this experience, or does anyone know of any reason that using alcohol-formalin (specifically Pen-Fix) would adversely affect IHC staining?

Thanks to all of you,

Diana Goodwin
Supervisor, Anatomic Pathology
Preston 6
215-829-6532
e-mail:  goodwind@pahosp.com