I have used NCL-DYS2 on pwax sections.( human and mouse were my tissues)
Around 1/100-200( TCS) using streptABC pxDAB system, citric acid M/W ph6. I
couldn't get 1 and 3 to work but.......it's definitely worth trying TRIS
pH10 M/W. This often gives positive results when citra returns neg result
carl
Carl Hobbs
Laboratory Manager
Molecular Neurobiology Group
MRC Centre For Developmental Neurobiology
New Hunts House
Guys Campus
Kings College London
SE1 1UL
020 7848 6810/fax 6816


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