[Histonet] RE: Blue counterstain for immunos
Hi Cathy,
How about the following simple trick:
Use the Dako hematoxylin 1:10 diluted in distilled water! Apply to your
FFPE section only for 15-20 seconds and rinse with tap water. Acetone-
fixed cryostat sections even shorter (or higher diluted). Check the wet
slide under the scope and judge if it needs perhaps another 15 seconds.
Success!
Chris van der Loos
Academical Medical Center
Dept. of Cardiovascular Pathology
Amsterdam - The Netherlands
From Cathy.Crumpton@tuality.org
Date Wed, 5 Nov 2003 12:48:42 -0800
To histonet@lists.utsouthwestern.edu
Subject [Histonet] Blue counterstain for immunos
Hi all. One of my pathologists saw an immuno stain that had a light
blue counterstain and, of course, he would like us to try "something
like that". Would anyone have any suggestions as to what kind of
counterstain would be good for the light blue? We use DAKO products
and other than hematoxylin and methyl green they did not have any
suggestions. We already use hematoxylin.
Thanks!
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