[Histonet] RE: Blue counterstain for immunos

From:"C.M. van der Loos"

Hi Cathy,
How about the following simple trick: 
Use the Dako hematoxylin 1:10 diluted in distilled water! Apply to your 
FFPE section only for 15-20 seconds and rinse with tap water. Acetone-
fixed cryostat sections even shorter (or higher diluted). Check the wet 
slide under the scope and judge if it needs perhaps another 15 seconds.
Chris van der Loos
Academical Medical Center
Dept. of Cardiovascular Pathology
Amsterdam - The Netherlands

From  Cathy.Crumpton@tuality.org 
Date  Wed, 5 Nov 2003 12:48:42 -0800 
To  histonet@lists.utsouthwestern.edu 
Subject  [Histonet] Blue counterstain for immunos 

Hi all.  One of my pathologists saw an immuno stain that had a light 
blue counterstain and, of course, he would like us to try "something 
like that".  Would anyone have any suggestions as to what kind of 
counterstain would be good for the light blue?  We use DAKO products 
and other than hematoxylin and methyl green they did not have any 
suggestions.  We already use hematoxylin. 

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