[Histonet] Finding GFP

From:Douglas A Gregg

I just went through this problem with and adenovirus expressing GFP. I
could not find it in PLP fixed paraffin embedded tissue. I had been able
to find a stronger signal by FA with a vesicular stomatitis virus-GFP.
However, I had great result with avidin biotin immunostaining with a MAb
to GFP made by Zymed at 1:75 with Dako antigen retrieval (citrate). I
used Vector red and got very strong red staining. I could see even more
of the weaker staining cells by using an FA scope and Texas red filters.
Good luck. This should make life easier.

Douglas Gregg
Plum Island Animal Disease Center
Greenport, NY 11944

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