Patsy,

My recollection is that we did lamin a/c immunofluorescence on frozen
sections of skeletal muscle (antibody from Chemicon), secondary Alexa dyes
from Molecular Probes, mount in Vectashield with DAPI. Excellent results

Ronnie

Ronnie Houston
Regional Histology Operations Manager
Bon Secours HealthPartners Laboratories
5801 Bremo Road
Richmond, VA 23226
(804) 287 7972
ronnie_houston@bshsi.com



-----Original Message-----
From: Gayle Callis [mailto:gcallis@montana.edu]
Sent: Wednesday, November 12, 2003 11:37 AM
To: pruegg@colobio.com; Histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] heart IHC work


Cut frozen sections, then fix for IHC.  Why compromise the tissue twice,
frozen then thaw, fix and process?  That is how I interpret this message,
correct? 



At 02:13 PM 11/11/2003 -0700, you wrote:
>Friends,
>I am involved in a project that requires doing IHC for Lamin A/C on heart
>tissue.  If anyone has experience with this please tell me all you know.
>Also, since most of this is DNA work they have pieces of fresh heart frozen
>at -70 dC.  What would be the best way to fix and process the frozen heart
>so that it would be well preserved for IHC.
>Thank you for advise in this matter
>Patsy
>
>
>_______________________________________________
>Histonet mailing list
>Histonet@lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology 
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)



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