RE: [Histonet] heart IHC work

From:"Houston, Ronnie"


My recollection is that we did lamin a/c immunofluorescence on frozen
sections of skeletal muscle (antibody from Chemicon), secondary Alexa dyes
from Molecular Probes, mount in Vectashield with DAPI. Excellent results


Ronnie Houston
Regional Histology Operations Manager
Bon Secours HealthPartners Laboratories
5801 Bremo Road
Richmond, VA 23226
(804) 287 7972

-----Original Message-----
From: Gayle Callis []
Sent: Wednesday, November 12, 2003 11:37 AM
Subject: Re: [Histonet] heart IHC work

Cut frozen sections, then fix for IHC.  Why compromise the tissue twice,
frozen then thaw, fix and process?  That is how I interpret this message,

At 02:13 PM 11/11/2003 -0700, you wrote:
>I am involved in a project that requires doing IHC for Lamin A/C on heart
>tissue.  If anyone has experience with this please tell me all you know.
>Also, since most of this is DNA work they have pieces of fresh heart frozen
>at -70 dC.  What would be the best way to fix and process the frozen heart
>so that it would be well preserved for IHC.
>Thank you for advise in this matter
>Histonet mailing list
Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology 
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)

Histonet mailing list


The information in this communication is intended to be confidential to the Individual(s) and/or Entity to whom it is addressed.
It may contain information of a Privileged and/or Confidential nature, which is subject to Federal and/or State privacy regulations.
In the event that you are not the intended recipient or the agent of the intended recipient, do not copy or use the information
contained within this communication, or allow it to be read, copied or utilized in any manner, by any other person(s).  Should
this communication be received in error, please notify the sender immediately either by response e-mail or by phone at 410-442-3250,
and permanently delete the original e-mail, attachment(s), and any copies. 

Histonet mailing list

<< Previous Message | Next Message >>