We simply run our known positive control for each stain or antibody protocol
and examine for optimal staining.I think you will find just that- the stains
are very nice. We are careful to use in house controls fixed and processed
just as our patient's specimens- in fact our controls ARE our patient's
specimens. We do the same for each new lot number.
 For ER and PR we did compare ten blocks that had previously been sent to
our reference lab for staining  against our in house stains before going
online with ER/PR. I also run many antibodies not in the Artisan menu  doing
the antigen retrieval and antibody incubation off the machine and using
Artisan ABC Detection. Artisan is a  lovely platform to use.

Jeff Silverman HT HTL QIHC
Pathologists" Assistant
Southside Hospital
Bay Shore NY USA


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