Re: [Histonet] URGENT! Questions regarding snap freezing in liquid nitrogen with isopentene

From:John Kiernan

There is abundant advice for you in
textbooks written 40 years ago (and
more recently too). ASK YOUR 
SUPERVISOR to suggest books or
review articles. 

For an honours-year project you should
be guided by your local boss and the
literature, not by answers from an
Internet listserver. Anyone can 
answer question. I could have answered
yours with all sorts of fake rubbish.
Would you have ueed it in your
honours dissertation?  
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London,   Canada   N6A 5C1
____________________________________________ wrote:
> Hi everyone,
> I am an Honours student, currently finishing up my Honours thesis.
> I am wondering if anyone could give me some REFERENCES on snap freezing mouse
> tissues using liquid nitrogen and ISOPENTENE. I have used liquid nitrogen ONLY
> for snap freezing my mouse tissues in my project, but not with isopentene. And
> i have been having cryostat sectioned mouse tissues that have a lot of tissue
> degeneration, as well as artefacts after fixing in 2% para & 0.5% Glu, and i
> got the idea from someone that this maybe due to the fact that i did not use
> isopentene in the snap freezing process.
> So what i want is to discuss this in my thesis. So it would be great if
> someone could give me any scientific references/comments/advice on this method
> whereby isopentene is used in the snap freezing process, and why using this is
> important.
> I have been doing some internet searching myself, but any advice or help from
> all histo experts out there would be greatly appreciated.
> Best regards,
> Hong Yuan Khor
> Flinders University
> South Australia
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