I have been asked to work up a procedure for doing direct immunofluorescence for collagen type IV alpha 5 chain on frozen section of skin.  Does anyone have any experience and/or a procedure, vendor, etc. for this?  Thanks for your help!
 
Martha Ward, MT (ASCP), QIHC
Molecular Diagnostics Lab
Wake Forest Universtiy Baptist Medical Center
Winston-Salem, NC 25157