Re: Oil Red O on formalin fixed tissue - frozen

From:Nancy E Weber

Hi Jane,
When I had to do an Oil Red O diagnostic stain on formalin fixed tissue, I'd
rinse the tissue in running water before embedding in the OCT.  After,  you
should let it air dry completely, then place the slide briefly in running
water to remove the OCT. You shouldn't stain the slide without removing OCT.
See if this helps your problem.  Nancy

Nancy Weber
Research Associate
Veterinary Clinical Sciences
Ohio State University
----- Original Message -----
From: "Jane Hosmer" 
Sent: Friday, November 08, 2002 2:06 PM
Subject: Oil Red O on formalin fixed tissue - frozen

> Dear Histos: Help!!!! I have many mouse tissues (livers,pancreas,muscle)
> fixed in 10% Buffered Formalin. These need to be frozen, then stained with
> Oil Red O. The problem I am running into is this: The stain is working,
> I am getting "blobs" of something staining also, that looks like it is
> between the tissue and the slide. It is not precipitate. Should I be
> washing the tissue thoroughly before I freeze in OCT???? I am picking up
> the section on +slides. Do I need to let them air dry before staining or
> wash them in H20? Any help would be appreciated.
> Jane Hosmer HT,HTL.
> UAB CPL Service Center
> Birmingham, Al.

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