RE: Oil Red O on formalin fixed tissue - frozen
I use formalin fixed tissue for my controls, and they usually work quite
nicely. I wash the tissue in running water for 1 hour before freezing, and
like you I use + slides. I do let them air dry before rinsing off the media
(they tend to stay on more reliably!). After staining in Oil red O, I would
try washing for 10 to 20 minutes in running water to help remove any excess
precipitate. Unfortunately ORO's do have a tendency to be blobby depending
on the density of the tissue.
Best of luck!
Tech II - Special Stains
Anatomic Pathology - Foothills Medical Center
Calgary Laboratory Services
Ph - 403-944-4760
Fax - 403-270-4093
From: Jane Hosmer [mailto:email@example.com]
Sent: Friday, November 08, 2002 12:07 PM
Subject: Oil Red O on formalin fixed tissue - frozen
Dear Histos: Help!!!! I have many mouse tissues (livers,pancreas,muscle)
fixed in 10% Buffered Formalin. These need to be frozen, then stained with
Oil Red O. The problem I am running into is this: The stain is working, but
I am getting "blobs" of something staining also, that looks like it is
between the tissue and the slide. It is not precipitate. Should I be
washing the tissue thoroughly before I freeze in OCT???? I am picking up
the section on +slides. Do I need to let them air dry before staining or
wash them in H20? Any help would be appreciated.
Jane Hosmer HT,HTL.
UAB CPL Service Center
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