RE: melanin on frozen sections
a quick method, either a Masson Fontana (microwave method), or Schmorl's
reaction would be able to give you a result in 5 to 10 minutes. As for
differentiating between melanin and melanocytes you may have better luck with
the DOPA oxidase reaction as it demonstrates cells capable of producing melanin
and not the melanin itself. The only down side is is is not quick ( 2-4
hours), and required careful temperature regulation and pH'd
Hope this helps!
I received a call from in a doctor in the dermatopathology clinic who
would like to stain for melanin on frozen skin sections. He is looking for
a rapid test as this would be done on MOH's patients. Preferably he would
like the stain to differentiate between melanin and melanocytes.
Any ideas would be appreciated!
University of Iowa Healthcare
Tech II - Special Stains
Anatomic Pathology - Foothills Medical
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