Re: B + T cell markers
Not that I am aware of - have not really looked at the lymphocytes in lungs.
Another antibody that you might think about is anti-mouse IgM - it is highly
expressed on Bcells.
Jenny Oblander wrote:
> Hi Barb,
> Have you had any problems with macrophage staining with the B220?
> Thanks Jenny
> -----Original Message-----
> From: Barbara Wright [mailto:bwright@gene.COM]
> Sent: Tuesday, November 12, 2002 11:22 AM
> To: Jenny Oblander
> Cc: Histonet (E-mail)
> Subject: Re: B + T cell markers
> Pharmingen CD45R/B220 is a good antibody for Bcells and DAKO CD3 is a good
> antibody for Tcells on FFPE mouse tissues. If you would like the protocols
> please let me know.
> Barb Wright
> Genentech, Inc
> Jenny Oblander wrote:
> > Hi Everyone,
> > I am looking for a good B+T cell antibody. It will be used on FFPE
> > mouse lung. If anyone has had good results, could you share the
> > clone,company and procedure. Thanks in advance, Jenny
> > J.Oblander, HT (A.S.C.P.)
> > Oklahoma Medical Research Foundation MS#32
> > 825 NE 13th St.
> > Okc,Ok 73104
> > email@example.com
> > 405-271-7534
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