RE: eNOS in eyes and Sihler stain
I have done eNOS/NOS Type III on rat vessels. The primary was from
Transduction Labs. I didn't need to do any AR, but it was crucial to make up
the antibodies in PBS/ 1% BSA/with calcium and magnesium. I made my life
easy and "procured" a bottle of premade PBS / Ca & Mg from the tissue
culture room :) I used primary dilution of 1:500 overnight at 4C, secondary
1:500 for 60 minutes at RT.
Good luck, if you would like a copy of my complete protocol let me know and
I'll forward it on to you.
Center for Cardiovascular Research
University of Rochester Medical Center
KMRB Room 2-9816
601 Elmwood Avenue.
PO Box 679
Rochester, New York 14642
> From: Cheryl Crowder
> Sent: Thursday, October 31, 2002 10:26 AM
> To: histonet
> Subject: eNOS in eyes and Sihler stain
> Good morning - I saw you question on the Histonet about a stain for nerve
> branching. I have found the directions and will send them to you by
> personal e-mail.
> Now, could you help me with my problem? I am starting a research
> project using eNOS I, II, III. The primary Abs are from Serotec and
> their help is minimal.
> Has anyonedone this type of IHC and what procedure did you use? The
> scant info I got did not mention antigen retrival and had a broad area
> for dilutions. Any help you can give me would "make my day". Thank
> you. Cheryl
> Cheryl Crowder, BA, HTL(ASCP)
> Chief Technologist
> Department of Pathobiological Sciences
> School of Veterinary Medicine
> Louisiana State University
> Baton Rouge, LA #160#70803
> (225) 578-9784
> FAX #160#(225) 578-9720
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