M:

We have been very happy with the polyclonal from Biodesign (B59136R). It
works well with Ficin, 10% Urea, and standard 10 mM Citrate Buffer antigen
retrieval.

Good luck with it!

Carlos

Carlos Genty
Breast Center Pathology
Baylor College of Medicine
Houston, Texas
cgenty@breastcenter.tmc.edu

-----Original Message-----
From: mrl0627@mail.ecu.edu [mailto:mrl0627@mail.ecu.edu]
Sent: Tuesday, November 12, 2002 10:15 AM
To: histonet@pathology.swmed.edu
Subject: anti-betagalactosidase in paraffin slides



Hello:
Has anyone had success with antibodies against B Gal in paraffin embedded
tissue?  We have tried several with no success and ICN does not recomment
the Cappel rabbit ab in paraffin.  I cannot use frozen sections (am working
with embryonic-early post-natal mouse inner ear and need sharp morphology.
Is there hope?  Thanks in advance for any assistance.
M.Loomer @ East Carolina University