RE: anti-betagalactosidase in paraffin slides

From:Carlos Genty


We have been very happy with the polyclonal from Biodesign (B59136R). It
works well with Ficin, 10% Urea, and standard 10 mM Citrate Buffer antigen

Good luck with it!


Carlos Genty
Breast Center Pathology
Baylor College of Medicine
Houston, Texas

-----Original Message-----
From: []
Sent: Tuesday, November 12, 2002 10:15 AM
Subject: anti-betagalactosidase in paraffin slides

Has anyone had success with antibodies against B Gal in paraffin embedded
tissue?  We have tried several with no success and ICN does not recomment
the Cappel rabbit ab in paraffin.  I cannot use frozen sections (am working
with embryonic-early post-natal mouse inner ear and need sharp morphology.
Is there hope?  Thanks in advance for any assistance.
M.Loomer @ East Carolina University

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