I took a QA class at the NSH this year, and in this class Peter Jackson and
David Blythe of the UK talked about "Nuclear Meltdown".  We were told that
doing heat induced epitope retrieval with buffer or even with water would
reverse this effect.  You cannot reverse the effect of "Nuclear Bubbling
Artifact" this same way, however.  It's worth a try!  Good luck!

Sarah A. Jones, HTL(ASCP)
Quality Assurance Histotechnologist
Richard-Allan Scientific
Kalamazoo, MI

-----Original Message-----
From: EPerry7701@aol.com [mailto:EPerry7701@aol.com]
Sent: Monday, November 11, 2002 2:38 PM
To: histonet@pathology.swmed.edu
Subject: Fwd: Nuclear pallor


Hi histonetters,

Our lab is having a problem. Our doctors have been noticing that the H&E 
stain slides of some of the GI biopsies have uneven (poor) nuclear staining.

We use a closed processing system and we clear with xylene. I have not
notice 
any condensation nor contamination on the inside of the lid nor the
reagents. 
I can not figure out what is going on. I have attached, with compliments of
a 
pathologist, a H&E slide.
It has only been notice with GI's and not with any other specimen.
Any suggestions will be greatly appreciated.

Genia
CCHS