RE: Artefact on IHC slides
|From:||Vinnie Della Speranza |
This is not an ihc artifact.
do you use sponges in your cassettes ? this looks to me that the biopsy
came into contact with dry sponges which caused drying artifact in the tissue
prior to immersion in fixative. the pattern of artifact is too regular to be
consistent with Glen's suggestion of adhesion loss.
if I am correct, any lab that uses those blue or white sponges in their
cassettes should saturate the sponges with formalin or whatever fixative you use
to avoid drying of the sample when it comes into contact with the needle
do let us know if you discover the cause. great mystery!!
Vinnie Della Speranza
Manager for Anatomic Pathology Services
University of South Carolina
165 Ashley Avenue Suite 309
>>> "Dawson, Glen" <GDawson@Milw.Dynacare.com> 10/30/02
My guess is that it is an adhesion
problem. Maybe the slide that the tissue
was put on had a piece of
gauze or something of the like sitting on it that
rubbed away the adhesive
properties of the slide in a definite pattern. In
the spots where the
adhesion was gone, the tissue lifted trapping DAB under
Wednesday, October 30, 2002 1:26 PM
Subject: Artefact on IHC
Please view pictures on:
histonet.org and click on View
List Serv Images
to view ipproblem1.jpg and ipproblem2.jpg.
is IHC CD20 on a biopsy specimen using DAB as the chromogen and
as the counterstain.
As far as I know, the tissue didn't come in contact
with gauze and the
artifact pattern isn't consistent with our tissue
cassettes. Just curious
as to any/all answers on this
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