Sentinel Node B32 Protocol Fixation Problem

From:"Cheasty, Sandra"

We are participating in a sentinel lymph node protocol (NSABP B-32) and are finding our lymph node sections not fixing very well. This only happens with lymph nodes for this particular protocol, all "regular" sentinel lymph nodes fix okay. I am having trouble finding out if they are being radioactively tagged differently or handled differently than "regular" s.n. cases.  We are currently using Rapid Fix in conjunction with a stir plate to facilitate fixation. 

Any ideas why this would be happening?


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