Two quick questions

From:Sara Kalla

1. In doing peroxidase anti-peroxidase staining on osteoclasts  in
culture, I've noticed that the controls stain positively (though not as
much) even after the addition of a H2O2 wash.  The larger, non-osteoclast
cells in the same culture (we started out with monocytes) do not stain in
the controls.  Has anyone else noticed this?  Any thoughts as to what's
going on/getting a cleaner stain?

2. Is there a list/flowchart of how media formulations are related out
there?  Specifically, I'm trying to figure out if/how alpha-MEM is related
to IMDM.

Sara Kalla

Blair lab
Dept. of Pathology
University of Pittsburgh Medical Center
Pittsburgh, PA

Science is what we do and magic is what they do.
			-Dr. K Brown, anthropology

<< Previous Message | Next Message >>