Two quick questions
1. In doing peroxidase anti-peroxidase staining on osteoclasts in
culture, I've noticed that the controls stain positively (though not as
much) even after the addition of a H2O2 wash. The larger, non-osteoclast
cells in the same culture (we started out with monocytes) do not stain in
the controls. Has anyone else noticed this? Any thoughts as to what's
going on/getting a cleaner stain?
2. Is there a list/flowchart of how media formulations are related out
there? Specifically, I'm trying to figure out if/how alpha-MEM is related
Dept. of Pathology
University of Pittsburgh Medical Center
Science is what we do and magic is what they do.
-Dr. K Brown, anthropology
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