Re: markers for arteries or veins
Good idea John.
You could also use IHC markers, even double staining, say CD31 for
endothelial cells and desmin for smooth muscle cells.
"J. A. Kiernan" wrote:
> On Wed, 28 Nov 2001, Judy Trogadis wrote:
> > We are interested in differentiating between arteries and
> >veins in the adult rat pulmonary vasculature. Are there specific
> > markers or antibodies we could use?
> Why not use an ordinary connective tissue stain
> such as van Gieson's. Arteries have thicker walls,
> mostly muscle (yellow) and veins have thinner
> walls with relatively less muscle and more
> adventitial collagen (red).
> > A fluorescent marker is required for the confocal
> > technique.
> It would be, but with van Gieson you could do
> the job with an ordinary microscope - much
> cheaper and easier to use! For what it's worth
> the acid fuchsine component of van Gieson is
> fluorescent. The picric acid suppresses
> autofluorescence, and so does the Weigert's
> iron haematoxylin that's used to stain nuclei.
> John A. Kiernan
> Department of Anatomy & Cell Biology
> The University of Western Ontario
> London, Canada N6A 5C1
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