Judy,
I use CD31 for marking endothelial cells in rats.  You can see my poster presentation on this by going to:  www.ihcrg.org
Patsy Ruegg

Judy Trogadis wrote:

> We are interested in differentiating between arteries and veins in the adult rat pulmonary vasculature. Are there specific markers or antibodies we could use? A variety of plant lectins exists which label endothelial cells but the literature is confusing about their specificities. They may also preferentially stain only certain organs.
>
> We are perfusing the rats, and cutting thick sections (~100 microns) on a vibratome for confocal microscopy. So staining could be carried out either at the time of perfusion or later, with the sections. A fluorescent marker is required for the confocal technique.
>
> I look forward to any suggestions.
> Thank you
>
> Judy
>
> Judy Trogadis
> Bio-Imaging Coordinator
> St. Michael's Hospital, 8Q
> 30 Bond St.
> Toronto, ON M5B 1W8
> Canada
>
> ph:  416-864-6060  x6337
> fax: 416-864-6043
> trogadisj@smh.toronto.on.ca