Re: Unstained Slide IHC Preservation
Great idea about dipping in paraffin, if it works for the block it sure
would for the slide.
----- Original Message -----
From: "Jenny Oblander"
To: "'Dawson, Glen'" ; "HistoNet Server"
Sent: Wednesday, November 21, 2001 11:36 AM
Subject: RE: Unstained Slide IHC Preservation
> I have used two methods in the past to retain slides. one is to freeze
> (only if you have unlimited space in your -20), or dip the slide in
> and store in a slide box, I have stored slides(coated with paraffin) for
> seven years and still have gotten excellent results from IHC. Jenny
> -----Original Message-----
> From: Dawson, Glen [mailto:GDawson@Milw.Dynacare.com]
> Sent: Wednesday, November 21, 2001 8:39 AM
> To: HistoNet Server; Dawson, Glen
> Subject: RE: Unstained Slide IHC Preservation
> I have a pathologist who wishes to preserve some unstained slides for
> Immunohistochemical analysis. The blocks must be returned so all he has
> work with is unstained. He estimates that it may be upwards of 10 years
> before the IHC would be done on these slides and would like to know the
> way to preserve the desired antigens on the slides. I have done IHC on 10
> year old blocks but never on such old unstained slides. Any suggestion on
> the best way to preserve these slides would be greatly appreciated.
> Thank-you in Advance,
> Glen A. Dawson BS, HT & IHC (ASCP)
> Lead IHC Technologist
> Milwaukee, WI
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