Re: Formula needed

From:Barry Rittman

It has been very quiet about your question and I think that is because anyone
who remembers this is showing their age.
We used to use Benedict's solution in 1950s to determine the presence of
reducing sugars in urine. It depended on reduction of copper ions.
Formula according to Dorland is
200 gms. sodium or potassium citrate,
200 gms. sodium carbonate,
125 gms. potassium sulfocyanate.
Dissolve in 800 ml. distilled water by boiling.
To this add a freshly prepared solution of 18 gms. copper sulfate in 100 ml.
distilled water.
Make final volume to 1 liter
For use
To 5 ml. in test tube add 8-10 drops fluid to be tested.
Boil 1-2 minutes.
The presence of a ppte red, yellow or green indicates the presence of
reducing sugars.
I am confused as to why you need to use this in a histology lab unless some
of the techs or pathologists are ODing on reagent grade sugars.

rueggp wrote:

> what is it supposed to be or do?
> patsy
> Adrian Biesecker wrote:
> > Hello all!
> >
> > I have a customer that is looking for the formula for Benedict's
> > solution?  I have never heard of this and cannot find it in our chem.
> > Data base.  If anyone knows the formula, I would really appreciate it!!
> > Thanks.
> >
> > Adrian Biesecker
> > BBC Biochemical
> > (800) 635-4477 x13
> >

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