cutting teeth

From:"Connolly, Brett M"

Histonetters,
I will likely have to paraffin embed, section and stain rat teeth and
mandible for a project. There will be some subsequent IHC involved also. We
plan to decal with EDTA. Any ideas/ caveats for making sure the teeth are
adequately decalcified. I suspect they will take longer than the cortical
bone, but they have to remain attached to the mandible. This is a new area
for me so please help with some guidance.
Thanks,
Brett   

Brett M. Connolly, Ph.D.
Merck Research Laboratories
Dept. of Pharmacology
WP26A-3000
PO Box 4
West Point, PA 19486
PH 215-652-2501
fax. 215-652-2075
e-mail. brett_connolly@merck.com






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