Re: Fibrinogen

I've had improved results with Dako polyclonal Fibrinogen #A0080 at 1:2500 
for 20 minutes. Then Dako's LSAB2 Link and Label (StrpAvd) for 10 minutes 
each before applying the chromogen. Place slides into 1% aqueous Cupric 
sulfate for 5 minutes and rinse before the counterstain. Control is placenta.

Eric C. Kellar
University of Pittsburgh Medical Center

Dear Histonetters:

I have recently been working with a DAKO polyclonal fibrinogen antibody in
formalin-fixed, paraffin-embedded tissue.  I am not having much success.
Does anyone out there have any experience with this or a similar antibody?
I'd appreciate any advice!


Jeanine Bartlett
Centers for Disease Control

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