Help with IHC/retrieval/veterinary

From:Gayle Callis <>

This article is worth its weight in gold for those involved with veterinary
IHC procedures:  

Optimization of immunohistochemical methods using two different antigen
retrieval methods on formalin fixed paraffin embedded tissues: experience
with 63 markers.

Ramos-Vara JA and Beissenherz ME, J Vet Diean Invest 12:307-311, 2000

You can write to authors and obtain a reprint if journal is not available:

Veterinary Medical Diagnostic Laboratory
University of Missouri
Columbia MO 65211

antibody, animal source, antibody source (vendor), clone, and pretreatments
(none, steamer, Proteinase K) and species stained are included.

Handy reference to have on hand.

Gayle Callis
Veterinary Molecular Biology
Montana State University
Bozeman MT 59717-3610
406 994-4705
406 994-4303

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