cytokine staining pattern in cells

From:Gayle Callis <>

Not interested in paraffin sections/antigen retrieved, only frozen
sections, and not formalin fixed, must be acetone or acetone/alcohol
fixation.  Interested in IFN gamma and IL4

Two questions:

what kind of staining pattern are you seeing with cytokine staining?
Perinuclear staining?  Many cells grouped in a pattern

Also, have you proven it is cytokine you have stained and not cross
reactivity of isotype matched control to cells within the tissue, eg
spleen?  and did you do ligand block or a specificity control to prove it
was cytokine?

Gayle Callis
Veterinary Molecular Biology
Montana State University
Bozeman MT 59717-3610
406 994-4705
406 994-4303

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