mouse monoclonal in mouse tissues

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From:Daniel Jimenez <>
To:HistoNet <>
Date:Wed, 26 May 1999 16:55:47 -0700 (PDT)
Content-Type:TEXT/PLAIN; charset=US-ASCII

I am currently using a mouse primary antibodies in mouse tissues, but I
have high background probably due to the inability of the
anti-mouse-secondary antibody to distinguish between the primary antibody
and the endogenous mouse Ig in the tissue. 
Does anybody know how to eliminate the backgroung problem without changing
the antibody? 

Thank you

Best Regards,

Daniel J

Daniel Jimenez
University of California - Davis
Dept of Pathology, Microbiology and Immunology
Bldg Haring Hall, Room # 1126
Davis, CA 95616
tel (530) 7521183

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