Hello Daniel,
In regards to 'mouse-on-mouse' IHC, there is a very simple solution:
InnoGenex produces an IHC kit, the "Iso Kit", which is designed 
specifically for mouse Abs on mouse tissue. The kit utilizes a pre-labeling 
system that eliminates any reaction with endogenous Ig - the technology is 
compatible with
with any mouse antibody, so you won't have to change your reagents. The 
results are very clean. I can send you the protocol.
Take Care,
Matthew Ogdie
IGX


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From: 	Daniel Jimenez[SMTP:dfjimenez@ucdavis.edu]
Sent: 	Wednesday, May 26, 1999 4:55 PM
To: 	HistoNet
Subject: 	mouse monoclonal in mouse tissues




I am currently using a mouse primary antibodies in mouse tissues, but I
have high background probably due to the inability of the
anti-mouse-secondary antibody to distinguish between the primary antibody
and the endogenous mouse Ig in the tissue.
Does anybody know how to eliminate the backgroung problem without changing
the antibody?

Thank you

Best Regards,

Daniel J

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Daniel Jimenez
University of California - Davis
Dept of Pathology, Microbiology and Immunology
Bldg Haring Hall, Room # 1126
Davis, CA 95616
tel (530) 7521183
email dfjimenez@ucdavis.edu
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